Because of the way light diffracts in a conventional microscope, a single fluorescent protein (FP) two nanometers in diameter will look like a blurry disk around 250 nanometers wide, says biophysicist ...
In recent years, single-molecule fluorescence spectroscopy has come of age. Single-molecule FRET (smFRET) serves as a 'spectroscopic ruler' 1 by reporting distance information between donor and ...
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